论文部分内容阅读
The development of a simple and user-friendly point of care test(POCT)device for quantitatively detecting and rapidly screening biomarkers in resource-limited settings presents a technical challenge.Most widely employed colorimetric test strips are qualitative methods.Although electrochemical techniques hold great promise for use in quantitative POCTs,the development of single-step electrochemical immunoassays remains a hurdle.In this study,we developed an intelligent microscale electrochemical device(iMED)for one-step,quantitative and multiplexed electrochemical detection of biomarkers for infectious diseases and tumors.A “plug-in-cartridge” technology was introduced and adapted for use in screen-printed electrodes(SPEs)in electrochemical devices.All reagents required for each immunoassay were preloaded into a plastic tube.Because these reagents were separated by spatially predefined air bubbles,they could be delivered automatically and rapidly to the surface of an SPE in one step under appropriate pressure.Using this iMED,we quantitatively detected biomarkers for two types of tumors and one infectious disease at sub-ng/mL levels in less than 30 min.We also demonstrated practical applications of this iMED by directly deploying it to detect total prostate-specific antigen(tPSA)in a small set of clinical samples,and our results were in excellent agreement with independent chemiluminescence assays conducted in local hospitals.This iMED represents a significant advance in the quantitative application of electrochemical detection in POCTs,and these POCTs show promise for the rapid detection of a wide range of molecular targets at low cost.