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Long-term nitrogen deposition has caused considerable perturbation called nitrogen saturation in forest system globally.Nitrogen saturation may affect significantly the fluxes of nitrous oxide and methane,but quantitative impact on these gas fluxes has not been fully understood yet.In this study,nitrous oxide and methane fluxes were investigated in three temperate forests of different stages of nitrogen saturation in central Japan.Our data indicate that the N2O emission was significantly enhanced with the development of nitrogen saturation.The results suggest that nitrification is stimulated in the surface soil due to increased nitrogen availability in a nitrogen-saturated forest to be a main factor that controls N20 emission.