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This study evaluated the effects of AS-IV on high glucose induced-gastric mucosal lesions compared to the effects of the Bu-zhong-yi-qi pill and the Xiaoke pill,which are already in clinical use.STZ-induced diabetic SD rats were treated with drugs for 12 weeks.Diabetes serum(DS)was used to mimic high glucose conditions in GES-1 cells in vitro at different concentrations and time points.The drugs were set at three concentration gradients for 24,48 and 72 h before being added to DS-induced GES-1 cells.The proliferation activity and inhibition ratio of cells were measured by the CCK-8 assay.Gastric tissues were examined by H&E staining.Cell morphology was observed by inverted phase contrast microscopy.Apoptosis of cells was detected by annexin V-FITC/PI.In addition,expres-sion levels of inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2)and mucin1(MUC1)were measured by Western blots.We found that the drugs significantly alleviated the pathological damage in STZ-induced gastric mucosal lesions.DS inhibited the viability of the cells in a dose and time-dependent manner,while pretreatment with drugs reversed these effects.Pretreatment with drugs also ameliorated the changes in cell morphology and inhibited cell apoptosis.Furthermore,the drugs decreased the expression levels of iNOS,COX-2 and MUC1.AS-IV showed the most beneficial effect compared with buzhong and xiaoke.These results suggest that AS-IV had a signifi-cant effect on high glucose-induce gastric mucosal lesions compares with buzhong and xiaoke.We speculate that this is in part through restoring the balance of iNOS,COX-2 and MUC1 expressions.Thus,AS-IV may be a potential antioxidant drug for treating diabetic gastropathy.