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It is now possible to slow down and trap a single molecule of double-stranded DNA (dsDNA) in a solid-state nanopore that is smaller in diameter than the double helix.This technique relies on a thorough understanding of the electromechanics of DNA in a solid-state nanopore ＜ 3.0 nm in diameter.By applying a voltage larger than the threshold for translocation, dsDNA can be forced to translocate through the pore.Once a current signature associated with a translocating molecule is detected, the electric field in the pore is switched to a value below the threshold, substantially reducing the translocation velocity from 1bp/10ns to ＞ 1bp/17μs.We can discriminate the difference between base pairs (AT/CG) with electrical current by trapping the biotinylated double-stranded DNA with streptavidin inside nanopores.