Method for liver tissue metabolic profiling based on liquid chromatography mass spectrometry

• 作 者: QiangHuang;PeiyuanYin;GuozhuYe;JingWang;JingChen;HongweiKong;XinLu;GuowangXu;
• 机 构: CAS Key Laboratory of Separation Science for Analytical Chemistry,National Chromatographic R&A Cente
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年期

　　Hepatocellular carcinoma (HCC) is one of the most frequent malignancy worldwide.Evaluation of tissue metabolites is with significant value in HCC study which can provide more direct information of metabolic disorder compared with biofluids.A protocol for the metabolic profiling of liver tissue was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS).According to the design of experiment (DOE), methanol/water (4∶1, v∶v) was selected as the optimal extraction solvent.The established method was validated with a linearity over the 10-5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986.The intra-day and inter-day RSD for most endogenous compounds were below 15% and the recovery of IS was from 84.8% to 109.1%.