【摘 要】
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Heavy metal pollution in the aquatic environment has increased in recent years, resulting in detrimental effects to aquatic animals.Here, we report the effect of Cd2+ on NAGase purified from the sperm
【机 构】
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Department of Veterinary Medicine, College of Animal Science, Fujian Agriculture and Forestry Univer
【出 处】
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全国农业生物化学与分子生物学第十四届学术研讨会
论文部分内容阅读
Heavy metal pollution in the aquatic environment has increased in recent years, resulting in detrimental effects to aquatic animals.Here, we report the effect of Cd2+ on NAGase purified from the spermary of Nile tilapia.The results showed that Cd2+ could reversibly inhibit the enzymatic activity of NAGase, and the IC50 was estimated to be 40.95 mM.Cd2+ was found to be a competitive inhibitor of NAGase, and an inhibition model was established by the substrate reaction method to study the kinetics of NAGase under inhibition by Cd2+.The inhibition constant was determined to be 17.13 mM.The microscopic rate constants of inactivation were also determined.
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