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Objective: Korean herbal formulation,Ojayeonjonghwan is used to treat male infertility and late-onset hypogonadism(LOH)symptoms including erectile dysfunction(ED).We investigated the antioxidant effects of modified Ojayeonjonghwan,KH-204 in androgen-deprived male rats.Methods: We investigated either distilled water(sham-operated)or leuprorelin 0.5 mg/kg,which was subcutaneously administered once to the back of rats.Male rats were divided into four groups(n=8 in each):a normal control group,an androgen-deprived control group and two androgen-deprived groups treated p.o.with either 200 or 400 mg/kg,KH-204 for 4 weeks.The testes and epididymides from rats in all groups were removed,weighed and subjected to histological examination and semen analysis after treatment.Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine(8-OHdG)and superoxide dismutase(SOD).Results: The results showed that KH-204 provided a protective effect against the androgen deficiency.When compared with an androgen-deprived control group,KH204 treatment significantly increased testis and epididymis weights,improved SOD activity,and raised plasma testosterone levels.It had a dose-dependent protective effect against DNA oxidative damage.Conclusion: This study suggests that the antioxidant effect of KH-204 contribute to the improvement of serum testosterone levels.It may be useful in inhibiting the induction of experimental andropause.