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Ultrasound and laser has been widely used for preclinical research.In this talk, we will present our recent works on the use of ultrasound and laser in two particular areas : photoacoustic based theranostic technologies and shear wave elasticity imaging of 3D cell culture systems.The photoacoustics based theranostic technologies are regarding the combination of ultrasound and photoacoustic/photothermal effects for tracking and delivery of gold nanoparticles (AuNPs).We demonstrated that the delivery of AuNPs can be greatly enhanced by combining molecular targeting and sonoporation.Also,molecular probes can be designed to track cell motion in 3D cell culture systems using dualwavelength optical resolution photoacoustic microscope (OR-PAM).In this talk,we will present the application of shear wave elasticity imaging to 3D cell culture systems as a tool for studying mechanobiology.3D cell culture systems have been used as an effective tool for biomedical research as they represent a more realistic model for cells compared to conventional 2D systems.In particular,spatial dynamics of extracellular matrix provides a wealth of information concerning the collective activities and intercellular crosstalk of adhering cells.We have developed shear wave elasticity imaging methods specifically for 3D cell culture systems.We will present our system setup, as well as experimental results on the relations between matrix stiffness and cancer cell behaviors.The potential in cancer mechanobiology will also be discussed.