Biochemical Analysis of Magnetic Immunoassay Using Functional Particles In Thin Channels

• 作 者: C.Bor Fuh J.R.Chan Y.C.Chang H.Y.Tsai
• 机 构: Department of Applied Chemistry, National Chi Nan University, Puli, Nantou 545, Taiwan
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年3期
• 关键词: Immunoassay Biochemical analysis Thin channel

　　Functional magnetic nanoparticles have been widely used in industrial and biomedical applications.Functional magnetic nanoparticles in a thin channel could potentially be a simpler and faster detection technique for biochemical analysis.This presentation would show biochemical analysis using functional magnetic nanoparticles in separation thin channels.In this method, magnetic nanoparticles labeled with antibody were flowed through a separation channel to form depositions on the bottom plate under magnetic fields for selective reactions with antigen.Determinations of biomarkers would be used as examples to demonstrate this detection method.The selectivity is higher than 92% and the running time is less than 15 min.In comparison with other methods, this method has lower detection limit and wider linear range.This technique has great potentials to provide a simple, fast, and selective analysis for particles and biochemical applications.