Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normo

• 作 者: BianjiangLiu;PengWang;ZengjunWang;YuejunJia;XiaobingNiu;WeiWang;WeiZhang;
• 机 构: Department of Urology,The First Affiliated Hospital of Nanjing Medical
• 出 处: 中国性学会性医学专业委员会第七次全国性医学学术会议
• 发表日期: 2011年期

Purpose:To analyze the abundance and difference of voltagedependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.Methods:High motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation.Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility.Results:Real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa.The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors.No significant