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Objective To evaluate mitral annulus dynamic morphology over the cardiac cycle in patients with mitral valve prolapse(MVP) by using real-time three-dimensional transesophageal echocardiography (RT-3D-TEE).Methods RT-3D-TEE was performed in 30 patients with MVP and 20 normal controls.And the mitral annular geometry and function changes throughout the cardiac cycle were analyzed by mitral valve quantification(MVQ) software.Results ①2-dimensional area of mitral annulus (A2D), 3-dimensional circumference of mitral annulus (C3D), anterior-to-posterior diameter of mitral annulus (DAP) and ellipsicity ratio of annulus diameter (E2D) were significant differences between the two groups.Anterolateral to posteromedial diameter of annulus (DALPm) and annulus height (H) were no significant differences between the two groups.②A2D, DALPm, E2D were significant differences between moderate MR and severe MR groups.③And the change rate of A2D, C3D, DAP, H, DALPm, E2D were no significant differences between the two groups throughout the cardiac cycles.Conclusions The mitral regurgitation in MVP patients has close relationship with the mitral annulus structure.