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Introduction Acupuncture especially is one of the most frequently requested of the complementary therapies in western countries turn.Electroacupuncture at Zusanli(ST36) could accelerate angiogenesis by up-regulating HIF-1 protein, and enhance skin microcirculation blood perfusion.In addition, it was used to antioxidant, which can inhibit the formation of superoxide.The paper aims to study how electroacupuncture at Zuansanli(ST36) effects on random-pattern flap survival rate through animal experiments.Materials and methods:Thirty female Sprague-Dawley rats were randomly divided into three groups: Control (no postoperative electroacupuncture) and Study A (postoperative electroacupuncture at non-acupoint areas, 10 Hz for 30 min per day) and Study B (postoperative electroacupuncture at Zusanli , 10 Hz for 30 min per day).The "McFarlane flap " rat models were established on the rat dorsum.After 7days electroacupuncture, the flap survival area ratio was measured.The tissue samples were taken for histological analysis.The VEGF, MDA and SOD expression were detected by immunohistochemistry.Result:7 days after operation ,the flap necrotic areas ratio in control group (66.65±2.81) %and in study A (64.65±2.42) % were significantly enlarged compared with that of the study B (48.81±2.33) % (P <0.01).Histological analysis demonstrated angiogenesis with mean vessel density per mm2 being lower in control group(15.4±4.4) and in study A (17.4±4.3) than in control group(27.2±4.1) (P <0.05).VEGF expression and SOD contents were significantly increased in study B Group compared with study A Group and control group (p < 0.01), whereas MDA level was reduced (p < 0.05).Conclusions:Electroacupuncture at Zuansanli(ST36) may increase the expression of VEGF, which can effectively increase angiogenesis to provide inadequate vascular perfusion.And block the release of ischemia-reperfusion injury-associated free radical, as well as the production of the superoxide anion.In addition, the up-regulated SOD and the down-regulated MDA are evidence for it.Above all, electroacupuncture at Zuansanli(ST36) can improve random flap survival rate effectively.