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A simple and efficient approach for the simultaneous separation and determination of catecholamines and indolamines by capillary electrophoresis with amperometric detection was developed.A fused silica capillary (45 cm × 50 μm i.d.) was dynamically coated with poly (diallyldimethylammonium chloride) (PDDA) modified gold nanoparticles (AuNPs) and employed for the separation of 3-indoxyl sulfate (3-IXS), vanillylmandelic acid (VMA), homovanillic acid (HVA), L-tryptophan (L-Trp), epinephrine (EP), normetanephrine (NMN), 5-hydroxytryptamine (5-HT), tryptamine (TA), 4-hydroxy-3-methoxybenzylamine (HMBA), 3,4-dihydroxybenzyl amine (DHBA) and isoproterenol (IP).The positively charged PDDA modified AuNPs formed a stable coating which significantly improved the selectivity of the solutes via ionic interaction.The effects of separation voltage, buffer pH and concentration were investigated.The coated capillary can be used for other biomarkers as well.Plasma aromatic and sulfur containing amino acids are good indicators of protein anabolism and catabolism, while blood reduced(GSH) and oxidized glutathione (GSSG) reflect oxidative status in an organism as a useful indicator of disease risk in humans.1 GSSG is usually found in very low concentrations in comparison to GSH in normal individuals.A method is developed by combining silica nanoparticles modified capillary electrophoresis with amperometric detection for the separation of GSH,GSSG and other amino thiols.