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Background : Previous studies have demonstrated that Th17 cells play critical role in immune related diseases,but lack of specific surface marker makes it difficult to achieve highly purified population of Th1 7 cells.The method to generate a highly enriched population will provide opportunities for studying the biology of Th1 7 cells in vitro and in vivo.Aims: To achieve highly purified population of Th17 cells by adjusting cytokines and methods of culture.Methods: CD4+、 CD44-naive T cells were separated from C57BL/6J mice by magnetic-activated cell sorting (MACS).