【摘 要】
：
Knowledge about protein interaction sites provides detailed information of protein-protein interactions (PPIs).To date,nearly twenty thousands of PPIs from
【机 构】
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中国农业大学生物学院,农业生物技术国家重点实验室,北京100193
【出 处】
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第七届全国生物信息学与系统生物学学术大会
论文部分内容阅读
Knowledge about protein interaction sites provides detailed information of protein-protein interactions (PPIs).To date,nearly twenty thousands of PPIs from Arabidopsis thaliana have been identified.Nevertheless,the interaction site information has been largely missed by previously published PPI databases.Here,AraPPISite,a database that presents fine-grained interaction details for A.thaliana PPIs is established.First,the experimentally determined 3D structures of 27 A.thaliana PPIs are collected from the Protein Data Bank database and the predicted 3D structures of 3,023 A.thaliana PPIs are modeled by using two well-established template-based docking methods.For each experimental/predicted complex structure,AraPPISite not only provides an interactive user interface for browsing interaction sites,but also lists detailed evolutionary and physicochemical properties of these sites.Second,AraPPISite assigns domain-domain interactions or domain-motif interactions to 4,286 PPIs whose 3D structures cannot be modeled.In this case,users can easily query protein interaction regions at the sequence level.AraPPISite is a free and user-friendly database,which does not require user registration or any configuration on local machines.We anticipate AraPPISite can serve as a helpful database resource for the users with less experience in structural biology or protein bioinformatics to probe the details of PPIs,and thus accelerate the studies of plant genetics and functional genomies.AraPPISite is available at http://systbio.cau.edu.cn/arappisite/index.html.
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