论文部分内容阅读
Rice blast,bringing a great harm in rice yield and quality,is one of the main diseases in rice.Pi21 is a significant gene with broad-spectrum resistance to rice blast.The mutation of Pi21 gene improves the blast resistance observably.In this study,we constructed a CRISPR/Cas9 vector,which contains two targets(Target1 and Target2)of Pi21 gene,and transformed to rice variety Nanjing9108.Twenty-eight T0 transgenic lines were obtained and confirmed by T-DNA specific PCR.