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Cysticercosis and taeniasis suis,caused by the pork tapeworm Taenia Solium,is one of the fatal parasitic diseases that affect both human and livestock.Our understanding on how cysticercus cellulosae develop into adult worm within human body at cellular and molecular levels is extremely limited due to inability to carry out in vivo experiments.In this study,we induced the in vitro differentiation of the cysticercus cellulosae pulling out its scolex with swine bile stimulation,evaluated the genome wide gene expression changes by the next generation high throughput sequencing RNA:seq,and performed transcriptome analysis.We have identified transcripts that are up:regulated and down:regulated at least two fold with statistic p:values less than 0.05 in responsive to bile stimulation.1201 genes were found to be up:regulated and 975 genes were found to be down:regulated in response to human bile stimulation.We observed 2318 genes that were not expressed either before or after bile activation.Interestingly,there are 149 genes that were expressed,but were completely shut off after bile activation.Likewise,there are 494 genes that were not expressed at all,their expression were observed only after bile activation.Gene ontology analysis,KEGG pathway mapping and manual annotations of regulated genes indicated that up: or down:regulated genes were involved in many pathways including cell cycle proliferation,apoptosis/cell death anddifferentiation,signal transduction and extracellular matrix (ECM),DNA replication and repair,general metabolic enzymes,metabolism,membrane components,and some transporters,protein degradation and mitochondrial functions.This work may serve as a starting point and experimental basis for further analysis and understanding the molecular regulation of tapeworm development,and provide insight into efficient drug target discovery and identification.