【摘 要】
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Objective To verify the anti-cancer effect of DCs induced by HMGB1 in vitro.Methods DCs were isolated from blood of normal volunteers and cultured to mature DCs under the induction of rHMGB1.DCs pheno
【出 处】
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中国抗癌学会肺癌专业委员会第十二届全国肺癌学术大会
论文部分内容阅读
Objective To verify the anti-cancer effect of DCs induced by HMGB1 in vitro.Methods DCs were isolated from blood of normal volunteers and cultured to mature DCs under the induction of rHMGB1.DCs phenotypes were analyzed by flow cytometry.CIKs were co-cultured with mature DCs for 14 days.The proliferation and cytotoxicity to A549 cells were investigated.Results DCs appeared mature morphological features under the microscope after 48 hours induction of HMGB1.DCs phenotypes analyzed by flow cytometry verified their maturation.CIKs proliferation was promoted during the co-culture with mature DCs.CIKs cytotoxicity to A549 cells was also strengthened.Conclusion HMGB1 could induce the maturation of DCs, and CIKs could achieve better anti-cancer effect by co-culture with mature DCs.
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