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Yellow seed color, which results from a thinner seed coat, is associated with improved feed quality of rapeseed meal and increased oil and protein contents.However, this trait follows various genetic models under different genetic backgrounds.To gain a better understanding of the genetic mechanisms underlying yellow seed color, a study was performed in two genetic backgrounds.The QTL analysis was undertaken using two populations derived from the crosses Quantum × no.2127-17 (HZ-1) and no.2127-17 × 94570 (HZ-2).In the HZ-1 population, three putative QTLs were detected in linkage groups N18, N5, and N3, respectively.For all of them, yellow seed color arose from the no.2127-17 alleles.Of these QTLs, the one in linkage group N18 (Bnsc -18a) behaved as a partially dominant yellow seed color gene and explained more than half of the phenotypic variation.In the HZ-2 population, three QTLs were found in linkage groups N9, N18, and N8, respectively.Of these QTLs, the one in linkage group N9 (Bnsc-9a) behaved as a completely dominant black seed color gene and explained more than half of the phenotypic variation.The QTL Bnsc-18a had a low seed color value and explained only 9.03-1.72% of the phenotypic variation in the HZ-2 population.Bulked segregant analysis of the extremes of a BC1 population derived from the cross no.2127-17 × 94570 (HZ-3) identified one QTL with a large effect that was identical to the QTL Bnsc-9a detected in the HZ-2 population.Four of the five QTL identified were population specific while one was mapped in two populations.These results show that different black-seeded forms had different seed color genes.Our findings also suggest that the use of multiple populations holds great promise for better understanding the genetic mechanism of seed color.