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Natural rubber latex (NRL), derived from the Hevea brasiliensis tree, is the main commercial feedstock for rubber and latex dipping industries.The surface nanostructure of Hevea brasiliensis natural rubber latex has been evidence by atomic force microscopy (AFM), confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).Base on atomic force microscopy analysis of the fresh natural rubber latex could estimate the thickness of the protein-lipid layer surrounding the rubber particle.The molecules in the particle were labeled with fluorescent Rhodamine (RB), and monitored by CLSM.SEM and TEM was been use to observe the surface of fresh natural rubber particle and dyed by osmium tetroxide.Fourier transform infrared spectroscopy (FTIR) has been used to characterization the nitrogenous groups in NR and deproteinized NR (DPNR).The FTIR and 1H-NMR analysis of phosphatase-treated DPNR confirmed that the presence of mono-and diphosphate terminations without phospholipids is also unlikely owing to the presence of a methylene proton signal of an isoprene unit linked to mono-and diphosphate groups.After treatment DPNR with lipase, the Mn, [η], and Higgins k were decreased.