【摘 要】
：
The joint toxicity of chlorantraniliprole, a novel insecticide that acts on ryanodine receptors, and sinigrin, a natural plant defense compound from brassicaceous vegetables, to the larvae of Spodopte
【机 构】
：
Department of Entomology, China Agricultural University, Beijing 100094, China;Biorational Pesticide
【出 处】
：
第二届生物农药理论探索及产业化技术创新研讨会
论文部分内容阅读
The joint toxicity of chlorantraniliprole, a novel insecticide that acts on ryanodine receptors, and sinigrin, a natural plant defense compound from brassicaceous vegetables, to the larvae of Spodoptera exigua (Hübner) was determined in this paper.Additionally, the joint effects of the two compounds on cytochrome P450 enzyme activity and on the expression levels of mRNA of three P450 genes (including CYP9A9, CYP6B, and CYP4G37) and an NADPH cytochrome P450 reductase gene (HQ852049) were investigated.The toxicity of the mixture of chlorantraniliprole and sinigrin to fourth-instar S.exigua larvae was 1.60-fold higher than the toxicity of the chlorantraniliprole-only treatment after 24 h.Induced by chlorantraniliprole and sinigrin, the specific activity of the P450 O-deethylase was affected in a time-, dose-, and organ-specific manner in fifth-instar S.exigua larvae.The effects were more pronounced in the midgut than in the fat body.The specific activity of the P450 O-deethylase in almost all treatments increased at 12, 24, and 36 h posttreatment compared with that in the control.Based on real-time PCR analyses, the expression levels of the P450 genes CYP9A9, CYP6B, and CYP4G37 and the NADPH cytochrome P450 reductase gene HQ852049 in fifth-instar S.exigua larvae were induced by chlorantraniliprole and sinigrin, and the trends were similar to the specific activity of the P450 O-deethylase.Therefore, the CYP9A9, CYP6B, and HQ852049 in the tested genes were the most inducible genes that were expressed when the S.exigua larvae were exposed to chlorantraniliprole and sinigrin.
其他文献
目的:本研究旨在优化同时蒸馏萃取法(SDE)提取山橙叶精油的工艺.方法:试验中选取山橙新鲜叶片为试验材料,采用同时蒸馏萃取法提取山橙叶精油,以加入NaC1量、水料比、浸泡时间、蒸馏时间为考察因素,以萃取率为考察指标,在单因素实验的基础上进行正交实验,同时用方差分析对其进行评价,找出各影响因素与萃取率之间的关系,优化提取工艺.结果:同时蒸馏萃取法萃取山橙叶精油的最佳工艺条件为浸泡时间8h、蒸馏时间6
毛茛科植物活性多样,本文主要对毛茛科植物具有农药活性的六个属:乌头属(Aconitum)、铁线莲属(Clematis)、黄连属(Coptis)、翠雀属(Delphinium)、白头翁属(Pulsatilla)和黑种草属(Nigella)进行了综述.其中六个属均有杀虫和抑菌活性;具有杀线虫活性的有黄连属和白头翁属;具有除草活性的有乌头属和黄连属;此外,白头翁属还具有杀螺活性.
目的：系统研究螺虫乙酯在不同类型的土壤中的吸附-解吸行为,探讨不同环境因子对螺虫乙酯在土壤中吸附行为的影响,进一步探讨其在土壤中的环境行为,为其在土壤中的环境风险和污染修复提供理论依据.方法：选择6种不同理化性质的土壤作为供试土壤,采用批量平衡法,研究螺虫乙酯在土壤中的吸附-解吸行为.结果与结论：研究表明,螺虫乙酯在6种土壤中的吸附-解吸过程符合Freundlich模型,6种土壤对螺虫乙酯的吸附能
目的：本文旨在通过比较我国不同地理种群的朱砂叶螨对现行的重要杀螨药剂的敏感性程度,为朱砂叶螨的防治提供理论支持.方法：实验选取重庆、北京、广州和广西地区的朱砂叶螨作为测定样本,通过采用叶片浸渍法,室内测定了朱砂叶螨对四种常见的杀螨(杀虫)剂一联苯肼酯、丁氟螨酯、螺螨酯和唑虫酰胺的敏感性程度.结果：联苯肼酯对朱砂叶螨重庆、北京、广州和广西地区种群的LC50值分别为24.76μg/mL、42.62μg
西瓜是夏季瓜菜的重要组成部分,近年来随着种植面积不断扩大,细菌性病害的发生日趋严重,对西瓜的产量和质量带来巨大影响.本文对发生在西瓜上的细菌性果斑病发生状况、致病机理及防治研究进展进行综述.
目的:小檗碱(Berberine)是一种常见的异喹啉生物碱,广泛应用于医药和农业领域.本实验主要研究小檗碱对拟南芥根尖分生细胞中生长素和细胞分裂素的影响.方法:本实验通过GUS染色法来研究小檗碱对DR5和CYCB1的影响.结果:0.5μg/mL,0.625μg/mL,1μg/mL,1.25μg/mL,1.5μg/mL,2μg/mL,2.5μg/mL,3μg/mL,4μg/mL,5μg/mL,6μg
目的：研究小檗碱及十七种衍生物的除草活性.方法：试验采用竖直培养皿法,以根长抑制率为指标,对小檗碱及其衍生物进行除草活性的测定.结果：对拟南芥根长抑制率最高的是小檗碱,为87.80％,衍生物中最高的是盐酸黄连碱,为76.56％,最低的是原阿片碱,为5.01％.结论：初步试验显示小檗碱对拟南芥根具有显著地抑制作用,其衍生物盐酸黄连碱和脱氢紫堇碱对拟南芥根有一定的抑制活性.
Azadirachtin, as a typical representative of botanical insecticides, which has the strongest antifeedant activity , is well-known in the world.Azadirachtin not only shows stable biological activity in
The fumigant, contact, and repellent activities of four essential oils extracted from Citrus limonum (Rutales: Rutaceae), Litsea cubeba (Ranales: Lauraceae), Cinnamomum cassia (Ranales: Lauraceae), an
Spodoptera exigua, a major pest of numerous cultivated crops in China, has been reported to develop serious resistance to many traditional chemical insecticides.A diet-incorporation bioassay was used