【摘 要】
：
Adeno-associated virus (AAV)-mediated gene transfer has been demonstrated to be an effective approach for treating Parkinsons disease (PD).Triptolide T10 is a monomeric compound isolated from triptery
【机 构】
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Department of Physiology and Key Laboratory of the Neurodegenerative Disorders of the Chinese Minist
【出 处】
：
中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Adeno-associated virus (AAV)-mediated gene transfer has been demonstrated to be an effective approach for treating Parkinsons disease (PD).Triptolide T10 is a monomeric compound isolated from tripterygium wilfordii Hook.f.(Thunder God vine), a traditional Chinese herb for anti-inflammatory medications.In the present study, we coadministered T10 with recombinant AAV2 in SH-SY5Y human neuroblastoma cells and in the striatum of C57BL/6 mice, and then evaluated the AAV-mediated gene expression levels.The results have shown that T10 significantly augmented the expression of AAV-mediated gene in a dose-dependent fashion without detectable cytotoxicity.As growing evidence indicated that inflammation contributed to the progression of PD, and the anti-inflammatory effect of T10 was shown in our previous studies, our data of T10 to enhance AAV transduction suggest that T10 might be potentially used as a facilitating reagent for the AAV gene therapy applications in neurodegenerative diseases.
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