SYP-5, a novel HIF-1 inhibitor, retards tumor cells invasion and angiogenesis

• 作 者: Xiao-RuiJiang[1]Li-HuiWang[1]XingLiu[1]Guo-LiangChen[2]Chun-FuWu[1]
• 机 构: Department of Pharmacology,Shenyang Pharmaceutical University,103 Wenhua Road,110016 Shenyang,PR Chi
• 出 处: 2015医学前沿论坛暨第十四届全国肿瘤药理与化疗学术会议
• 发表日期: 2015年期

　　Objective Hypoxia-inducible factor-1 (HIF-1) plays an essential role in carcinogenesis.The overexpression of HIF-1 induced by hypoxia is closely associated with metastasis, poor prognosis and high mortality.In this study, the effect of a novel compound SYP-5 on HIF-1 and HIF-1 mediated cell invasion and angiogenesis were examined.Methods In this study, the luciferase reporter assay was used to detect the ability of SYP-5 to inhibit the HIF-1 activity.We performed western blots to evaluate the antitumor effect of SYP-5 targeting HIF-1.Moreover, the proteins of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMP)-2 that are targets of HIF-1, were also examined.Furthermore, the Transwell, RTCA and tube formation assays were used to investigate the effect of SYP-5 on cell invasion and angiogenesis.