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A reproducible protocol for somatic embryogenesis was established for the ornamental ginger Hedychium coronarium using filaments.Callus was induced on MS medium supplemented with 4 mg · L-1 2,4-D,4 mg ·L-1 NAA,1 mg · L-1 6-BA.Embryogenic calli were selected and cultured on somatic embryo induction medium containing MS basal salts,B5 vitamins,100 mg · L-1 glutamine,230 mg · L-1 proline,100 mg · L-1 malt extract,0.25 mg · L-1 NAA,0.5 mg · L-1 TDZ,45 g · L-1 sucrose for 30 days,then transferred on MS medium free hormone.Mature somatic embryos were obtained after 40 days culture.About 50~60 somatic embryos were obtained from per gram callus and 85%of them germinated into plantlets.Regenerated plantlets were transferred on half strength MS medium with 1 g · L-1 active charcoal to promote development.Well rooted plantlets were successfully acclimatized with a survival rate of 90%.Six tetraploids(4n = 68)were selected from 100 regenerated plants and ploidy levels were determined by flow cytometry and chromosome counting.Variation in the morphological characteristic was found between diploids and tetraploids under the same growing conditions.The stomata sizes of the tetraploid were significantly larger than those on the diploid counterparts,while the frequency of stomata was significantly reduced.Moreover,tetraploid plants developed larger flowers and with stronger aroma,all contributing to higher ornamental value of Hedychium coronarium.