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The ability to knock down expression levels of an endogenous gene product and to monitor effects on individual cells or whole animals by RNAi has emerged as a powerful strategy for rapid analysis of specific gene function.Here a novel inducible RNAi system which relies on a chemical switch is introduced.This system has addressed the major issues existing in current transient and constitutive RNAi approaches that cause either poor genotype/phenotype consistence or cell lethality.Based on conditional loss-of-function genetic screen we have identified a series of genes which are involved in virus replication,tumor growth or drug resistance.The feasibility for developing those genes as potential drug targets is also assessed.