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Heat-shock proteins can be expressed in response to a variety of adverse environmental infection.In the present study,the cDNA sequence of Hyriopsis cumingii HSP70(hcHSP70)was cloned by 3′rapid amplification of cDNA ends methods(3′-RACE)based on a long chain sequence of hcHSP70 and was determined by high flux sequencing for transcriptome of H.