【摘 要】
：
Y5247 (development code No.), a quinone-outsider-inhibitor (QoI), is a novel fungicide with a broadspectrum of fungicidal activity against plant filamentous fungi, including B.cinerea.Compared to azox
【机 构】
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Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Education,
【出 处】
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中国植物病理学会化学防治专业委员会第九届中国植物病害化学防治学术研讨会
论文部分内容阅读
Y5247 (development code No.), a quinone-outsider-inhibitor (QoI), is a novel fungicide with a broadspectrum of fungicidal activity against plant filamentous fungi, including B.cinerea.Compared to azoxystrobin,Y5247 had a stronger inhibitory effect on mycelial growth of B.cinerea.The baseline sensitivity of 100 strains showed the EC50 values for Y5247 inhibiting mycelial growth of B.cinerea ranged from 0.003 3 ~ 0.392 1 μg/mL with a mean of (0.085 9 ± 0.078 8) μg/mL and the frequency distribution of EC50 values was unimodal.Mutants with stable resistance to Y5247 were not obtained following exposure to ultraviolet light or chemical mutagenesis.However,strains resistant to Y5247 were collected from greenhouses where other QoI fungicides had frequently been sprayed to control gray mold of strawberry.Among the Y5247-resistant strains, EC50 values for Y5247 and other QoIs, including azoxystrobin, kresoxim-methyl and pyraclostrobin, were positively correlated.No cross-resistance was detected between Y5247 and the other fungicides with different modes of action such as carbendazim, diethofencarb, boscalid,fluazinam, procymidone, and fludioxonil.The Y5247-resistant strains showed comparable fitness in mycelial growth,sporulation and pathogenicity to that of Y5247-sensitive strains.The risk of B.cinerea developing resistance to Y5247 was assessed as high.Analysis of cytb in B.cinerea showed that all the Y5247-sensitive and resistant strains carried the type Ⅱ cytb (which lacks the intron Bcbi-143/144) and the point mutation G143A occurred in all the resistant strains tested.
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