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In this talk, I will summarize the recent research on synthesis, characterization and applications of 2D semiconducting nanomaterials in my group [1].I will introduce the synthesis and characterization of novel 2D semiconducting materials, such as graphene [2], MoS2, WS2, TiS2, TaS2, etc.[3], especially the first-time synthesized hexagonal-close packed (hcp) Au nanostructures on graphene oxide [4].Then I will demonstrate the applications of the 2D nanomaterials in chemical and bio-sensors [5], solar cells [6], electric devices [7], memory devices [8], conductive electrodes [5b,e,f,h 6a-b, 7a, 8a-b, 9], cell cultures [10], matrix of MALDI-TOF-MS [11], supercapacitors [12] etc.