Screen and identify the critical proteins of central nervous system hemangioblastoma tumor cells usi

• 作 者: RongXIE[1]Jian-HongZHU[1]HangLIU[2]Hui-ZhiFAN[2]JunYAO[2]XingWU[1]Lu-PingCHEN[1]Yi-WenSHEN[1]Peng-YuanYANG[2]
• 机 构: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China
• 出 处: 中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
• 发表日期: 2007年期

　　Objective We carried out a pilot study in which we analyzed the proteomes of central nervous system heman-gioblastoma (CNS HB) tumor cells by using on-line capillary 2D-HPLC combined with LTQ-Orbitrap ESI MS so as to screen and identify the critical proteins related to CNS HB occurring and investigate the histological origin and pathogenesis of the disease.Methods CNS HB cells were cultured and identified by enzyme digestion from CNS HB tissues collected during operations.Normal human nerve cells were obtained as control.Total proteins of cells were extracted and enzymolysed.