【摘 要】
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Background Clinical use of bioartificial liver (BAL) strongly relies on the development of human liver cell lines.The aim of this study was to establish and assess the potential usage of the HepG2 cel
【机 构】
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02 Military Hospital of China
【出 处】
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中华医学会第十六次全国病毒性肝炎及肝病学术会议
论文部分内容阅读
Background Clinical use of bioartificial liver (BAL) strongly relies on the development of human liver cell lines.The aim of this study was to establish and assess the potential usage of the HepG2 cell line stable expressing human Augmenter of Liver Regeneration (hALR).Methods The cDNA encoding hALR protein was inserted into pcDNA3.1 to generate pcDNA3.1/hALR.Then pcDNA3.1/hALR was transfected to HepG2 to establish cell line that stable expressed hALR (HepG2 hALR).Laboratory-scale BAL bioreactors were loaded with 800 million HepG2 hALR cells and cultured for 4 days.Parameters of hepatocyte specific function and general metabolism were determined.Results The cell line that stable expressed human ALR was successful established.The expression of recombinant hALR increased in the HepG2 hALR than that in HepG2 detected by immunofluorescence assay and immunoblot.In BAL bioreactor, HepG2 hALR produced significant higher α-fetoprotein (AFP) (127.3％ of HepG2, P＜0.05), urea (128.8％ of HepG2, P＜0.05) and eliminated glucose (135.7％ of HepG2, P ＜ 0.05) on day 4.The level of human albumin was 117％ of that in HepG2, whereas the difference showed no significance (P ＞ 0.05).After cultured for 24 hours, the mean lidocaine remove rate was 65.1％ and 57.3 ％ in culture supernatant of HepG2 hALR and HepG2, respectively (P ＜0.01).Conclusions HepG2 hALR cells showed liver-specific functionality when cultured inside the bioreactor and would be a potential cell source for BAL.
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