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Apicomplexan parasites infect nearly all vertebrates including humans and cause some severe diseases such as malaria,toxoplasmosis,cryptosporidiosis and coccidiosis.Coccidiosis is responsible for substantial economic losses in livestock and poultry industries.Like most intracellular pathogens,egress from host cells is a vital step of the intracellular lifE-cycle of apicomplexan parasites,which have attracted attentions from many research groups.Previous studies have shown that ethanol could stimulate the releaise of certain protein contents from micronemes by elevating intracellular Ca2+ concentration ofToxoplasma gondii.Release ofmicroneme proteins is a vital step for tachyzoites invasion and egress of host cells.However,little information on egress is known on Eimeriaspecies which are the pathogens causingcoccidiosis.In this report,some types of short chain alcohols were used to study the egress of Eimeriafor the first time.These alcohols were incubated with Eimeriatenallasporozoites:infected primary chicken kidney cells and the egress rates were calculated through flow cytometry analysis after treatment.The results showed that,compared to control group,only ethanol and isopropanol could induce premature egress of E.tenallasporozoites from infected cells,with slight host cell damage and the egress was hypothesized to be affected both by developmental stages of sporozoites in infected cells and inositol triphosphate pathway (IP3) of the parasites.Taken together,our study is developing a novel approach toin vestigate the mechanism of egress ofEimeria,a new orientation to study the relationship between intracellular pathogens and their hosts.