Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-

• 作 者: Xiaona Zhao
• 机 构: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Tai'an 271018,
• 出 处: 华东区中兽医研究会第二十三届学术研讨会
• 发表日期: 2017年4期
• 关键词: Yu-Ping-Feng polysaccharide active molecules immunological activity chicken macr

　　Yu-Ping-Feng (YPF) is a well-known traditional Chinese multi herbal formula,and it is frequently being used to treat cold and flu and inflammation-associated diseases.We aim to evaluate the immunostimulatory activity of polysaccharide from Yu-Ping-Feng (YPF-PS) in vitro on chicken macrophage to make clear the bioactive molecular of Yu-Ping-Feng.YPF-PS was performed gel permeation chromatography (GPC) and gas chromatography?mass spectrometry (GC-MS) analysis,amino acid analysis and nuclear magnetic resonance (NRM) spectroscopy to elucidate its structure and screen the immunomodulatory components.In order to evaluate the effect of YPF-PS on immunology,lipopolysaccharides (LPS)-induced cell proliferation and phagocytosis rate and the levels of cytokine: nitric oxide (NO),tumor necrosis factor alpha (TNF-α),interleukin-1 beta,interleukin-6 (IL-1 β,IL-6),interferon beta (IFN-β) and costimulatory molecules CD80,CD86 in chicken macrophage were detected.The result indicated that molecular weight the weight of YPF-PS was distributed on 5 KD~500 KDa,which is a mixture of glucose (Glu),fructose (Fru),arabinose (Ara) and few rhamnose with the molar ratio of 61.29: 55.47: 99.67: 19.58,having anomeric carbon signals of β-L-arabino furanoside,α-L-rhamnoside,α-D-glucopyranoside and β-D-fructofuranose.Furthermore,cell immunology experiments show that YPF-PS at dosage of 15.62 μ gmL-1 could increase the LPS-induced cell proliferation and phagocytosis rate significantly in chicken macrophage,moreover,the levels of cytokine:NO,TNF-α,IL-1 β,IL-6 and IFN-βin cells elevated obviously,equally shocking,costimulatory molecules CD80,CD86 were detected having a tremendous enhancement.We believe that the YPF-PS has potent immunomodulatory properties and could be explored as a novel potential immunostimulant for the food and pharmaceutical purpose.