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Antibiotic therapy has been successfully used in the treatment of various infectious diseases caused by bacteria.But antibiotics can get a long-term change of human gut microbiota[1].In this study, we establish a standardized model of gut dysbacteria mice, according to the characteristics of human who take antibiotics.We intend to find out the characteristics and mechanism of cephalosporins antibiotics effecting on intestinal microbiota, in order to provide more reasonable scientific basis to the future clinical application of antibiotics.