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The Sesame-flavor Liquor is one of innovative liquor types in China, which has been universally popular for its technology characteristics combined with multifold-flavor liquors and elegant sesame flavor.High temperature Daqu is a sacchariferous starter and it can provide large amounts of microorganisms, enzymes, flavor components and its precursors for brewing sesame-flavor liquor.So its quality is directly relevant to the output rate and quality of liquor.Molecular biological and molecular ecological technologies were firstly used to reveal the bacterial community diversity from high temperature Daqu of Sesame-flavor liquor manufactured by Bandaojing Co.Ltd.Further, the results can also supply scientific data for study of the functional microbes mechanism.