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Two undescribed pyrrolizidine alkaloids were isolated from the herbs of Crotalaria albida,named neocroalbidine and neocroalbidinone.Their structures were established mainly by means of a combination of NMR spectroscopic and mass spectrometry techniques.The compounds were exhibited any significant cytotoxic activity against six tumor cell lines (A375,HepG2,7721,Hep3B,Hela and primary mouse hepatocytes) in vitro.