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1990年和1991年从湖南省8个地区(市)、25县(市)烟区调查采样,发现湖南烟草病毒病田间症状表现有7种类型,以花叶症状最多.通过鉴别寄主反映和A 蛋白酶联检测,初步确定湖南烟草上存在CMV,TMV,PVY 和TRSV 4种病毒的感染.它们除单独侵染外,还有TMV+CMV,TMV+PVY2种复合侵染类型,其中以CMV 侵染频率最高,达53.08%.应用A 蛋白酶联法检测314个花叶病样本,结果表明湖南烟草花叶病主要由CMV,TMV 侵染所致,其中又以CMV 侵染频率最高,达67.84%.
In 1990 and 1991, samples were collected from 8 districts (cities) and 25 counties (cities) in Hunan Province, and found that there were 7 types of symptom in the field of tobacco virus disease in Hunan Province, with the most mosaic symptom. By identifying host and A Protease assay was used to detect the presence of four CMV, TMV, PVY and TRSV viruses in Hunan tobacco.These two types of combined infection were TMV + CMV and TMV + PVY, with CMV infection The frequency was the highest (53.08%). A mosaic enzyme linked immunosorbent assay (ELISA) was used to detect 314 mosaic virus samples. The results showed that Hunan mosaic virus mosaic virus was mainly caused by CMV and TMV infection, and CMV infection reached the highest frequency of 67.84%.