论文部分内容阅读
以聚乙烯醇缩丁醛为固定葡萄糖氧化酶 (GOD)的载体 ,将GOD附在铂丝电极上并采用高氯酸·三_2,2′_联吡啶合钴(Ⅲ)[Co(bpy)3(ClO4)3]作为电子媒介体制得了电流型葡萄糖酶电极。讨论了溶解性媒介体Co(bpy)3(ClO4)3 的浓度、溶液的 pH值和温度对该电极电流响应的影响。该介体型葡萄糖传感器在优化的实验条件下 ,对葡萄糖表现出良好的响应特性 ,如响应快、重复性和稳定性好 ,传感器线性范围为6.0×10 -6～1.1×10 -4mol/L ,检出限为3.0×10 -6mol/L。将该电极用于人血清中葡萄糖测定 ,其结果与传统方法测得的结果一致。
Using polyvinyl butyral as a carrier for immobilized glucose oxidase (GOD), GOD was attached to a platinum wire electrode and the perchloric acid tris-2,2’-dipyridyl cobalt (III) [Co ) 3 (ClO4) 3] as an electron mediator, a current-type glucose enzyme electrode was obtained. The effects of the concentration of Co (bpy) 3 (ClO4) 3, the solution pH and the temperature on the electrode current response were discussed. The glucose sensor showed good response to glucose under the optimized experimental conditions, such as fast response, good repeatability and stability. The linear range of the sensor was 6.0 × 10 -6 ~ 1.1 × 10 -4 mol / L, The detection limit was 3.0 × 10 -6 mol / L. The electrode was used for the determination of glucose in human serum, and the result was in agreement with that of the conventional method.