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目的建立同时测定田基黄中4种黄酮(异槲皮苷、槲皮苷、槲皮素-7-O-α-L-鼠李糖苷和槲皮素)含量的RP-HPLC法。方法采用Zorbax SB C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-体积分数为0.5%的乙酸溶液为流动相进行梯度洗脱,检测波长为360 nm,流速为1.0 mL.min-1,柱温为35℃。结果异槲皮苷、槲皮苷、槲皮素-7-O-α-L-鼠李糖苷和槲皮素质量浓度分别在6.09~203.00、18.93~631.00、1.89~63.00、4.11~137.00 mg.L-1内与峰面积线性关系良好,4种黄酮低、中、高的回收率为97.5%~102.6%,RSD均小于3%。结论 RP-HPLC法可作为田基黄质量控制的一个有效方法 。
OBJECTIVE To establish an RP-HPLC method for the simultaneous determination of four flavonoids (isoquercetin, quercitrin, quercetin -7-O-α-rhamnoside and quercetin) in Tianjihuang. Methods The gradient elution was carried out on a Zorbax SB C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-0.5% acetic acid as the mobile phase. The detection wavelength was 360 nm and the flow rate was 1.0 mL · min-1 The column temperature was 35 ° C. Results The isoquercitrin, quercitrin, quercetin -7-O-α-L-rhamnoside and quercetin were 6.09 ~ 203.00, 18.93 ~ 631.00, 1.89 ~ 63.00 and 4.11 ~ 137.00 mg, respectively. The linear relationship between L-1 and peak area was good. The recoveries of low, middle and high flavonoids were 97.5% -102.6% and RSD were less than 3%. Conclusion RP-HPLC can be used as an effective method for the quality control of Tianji yellow.