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目的建立肺炎支原体感染的金黄地鼠动物模型,且对动物模型肺组织进行病理检查和血清粒细胞集落刺激因子(G-CSF)的检测,对G-CSF在呼吸道感染性疾病中的病原诊断作用进行研究。方法用肺炎支原体国际标准株(Mp-FH)经鼻感染金黄地鼠,经2、4、6、8、10周,获取肺脏病理标本,同时检测动物血清G-CSF。结果15只金黄地鼠肺炎支原体(Mp)肺炎动物模型肺的病理均呈炎性改变,其血清的检测结果显示G-CSF均呈阳性,而对照组5只鼠检测结果均呈阴性。结论金黄地鼠对Mp易感,在Mp感染金黄地鼠时血清G-CSF明显升高,其发生机制有待于进一步研究;测定血清G-CSF也可作为感染的辅助诊断指标之一。
Objective To establish an animal model of Mycoplasma gondii infection of golden hamster and to examine the pathological features and serum G-CSF in lung of animal models and to evaluate the pathogenicity of G-CSF in respiratory tract infectious diseases research. Methods The golden hamster was infected by Mycoplasma pneumoniae (Mp-FH) nasally and the pathological specimens of the lungs were obtained at 2, 4, 6, 8, and 10 weeks after infection. Serum G-CSF was also detected. Results The pathology of the lungs of 15 golden hamster mice with M pneumoniae pneumonia showed inflammatory changes. The results of serum tests showed that G-CSF was positive, while the control rats had negative results. Conclusions The golden hamster is susceptible to Mp. Serum G-CSF is obviously increased when Mp is infected with golden hamster, and its mechanism needs to be further studied. Serum G-CSF can also be used as an auxiliary diagnostic index for infection.