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班氏丝虫:从冈比亚按蚊或埃及伊蚊获得班氏丝虫坦桑尼亚株感染期幼虫,采用各种途径接种17只雌雄沙鼠,其中10只腹腔内接种100条幼虫,5只皮下接种20~100条,2只将40条幼虫直接种入腘窝淋巴结。分别于感染后5小时至154天解剖。沙鼠麻醉时进行心腔穿刺,血液经微孔滤器过滤检查微丝蚴,按Ash等描述方法解剖砂鼠,注意搜索淋巴系统、睾丸及心、肺,接种后48小时,未找到发育或存活的幼虫;1只沙鼠于感染后5小时从腹腔找回40%幼虫,但其表皮均为细胞所吸附,24小时后,仅发现为细胞胶质团所完全包统的少数幼虫;其余16只沙鼠均为阴性。
Bancroftian filariasis: Infectious larvae of Bancruncias strain Tanzania were obtained from Anopheles gambiae or Aedes aegypti and 17 male and female gerbils were inoculated by various routes, 10 of which were inoculated intraperitoneally with 100 larvae and 5 were subcutaneously inoculated with 20 ~ 100, two of 40 larvae directly into popliteal lymph nodes. Five hours to 154 days after infection were dissected. Gerbils were perfused with cardiac cavity during anesthesia. The blood was filtered through a microfilter to examine microfilaria. Anatomy of the gerbils was performed as described by Ash et al. The search was for lymphatic system, testis and heart, lungs, and no development or survival was found 48 hours after inoculation Of the larvae. One gerbil recovered 40% of the larvae from the abdominal cavity 5 hours after infection, but the epidermis was all adsorbed by the cells. Only 24 hours later, only a few larvae completely encapsulated by the cell glomerulus were found. The remaining 16 Only gerbils were negative.