【摘 要】
：
A recessive epistatic genic male sterile (REGMS) two-type line, 7365AB (Bnms3ms3ms4msRrfRf/BnMs3ms3ms4ms4RfRf), combined with the fertile interim-maintainer 7365C (Bnms3ms3ms4ms4rfrf) is an effective
【机 构】
：
National Key Laboratory of Crop Genetic Improvement,National Sub-center of Rapeseed Improvement in W
【出 处】
：
中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
论文部分内容阅读
A recessive epistatic genic male sterile (REGMS) two-type line, 7365AB (Bnms3ms3ms4msRrfRf/BnMs3ms3ms4ms4RfRf), combined with the fertile interim-maintainer 7365C (Bnms3ms3ms4ms4rfrf) is an effective pollination control system in hybrid rapeseed production.Here, we report an effective strategy used to fine map BnMs4 and BnRf.The two genes were both defined to a common region microsyntenic with Arabidopsis chromosome 3 using intron polymorphism (IP) markers developed according to Arabidopsis genome information and published genome organization comparisons of the A genome and Arabidopsis.The near-isogenic lines (NILs) 7365AC of BnRf and 736512AB of BnMs4 were constructed to screen developed markers and create genetic linkage maps.Nine polymorphic IP markers (P1-P9) were identified.Of these, P2, P3, P4, and P6 were linked to both BnMs4 and BnRf with genetic distances less than 0.6 cM.Three simple sequence repeat (SSR) markers, SR2, SR3, and SR5, were also identified by using public information.Then, all markers linked to the two genes were used to compare the micro-collinearity of the regions flanking the two genes with Brassica rapa and Arabidopsis.The flanking regions showed rearrangements and inversion with fragments from different Arabidopsis chromosomes, but a high collinearity with Brassica rapa.This collinearity provided extremely valuable reference in map-based cloning in polyploidy Brassica species.Those IP markers will contribute to identifying variations within and among Brassica species, providing convenience for molecular marker-assisted selection (MAS) breeding, accelerating the process of gene cloning, and providing more direct evidence of multiple alleles between BnMs4 and BnRf.
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